Application of Soluble and Immobilized Α - Galactosidase from Acinetobacter Sp for Degradation of Galacto-oligosaccharides in Cowpeas

نویسنده

  • LAKSHMI NARASU
چکیده

α-galactosidase purified from Acinetobacter sp. was immobilized in four different matricesCalcium alginate, Gelatin, Agarose and k-Carrageenan. On comparative analysis, k-Carrageenan immobilized enzyme showed highest immobilization yield of 68% and storage stability for 30 days. Gelatin showed an immobilization yield of 57%. Soluble enzyme led to decrease of 84% in raffinose and 76% in stachyose concentration in cowpeas whereas k-Carrageenan immobilized enzyme led to decrease of 72% of raffinose and 54% of stachyose thereby decreasing the flatulence to greater extent.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Immobilization of the Antarctic Bacillus sp. LX-1 α-Galactosidase on Eudragit L-100 for the Production of a Functional Feed Additive

Partially purified α-galactosidase from Bacillus sp. LX-1 was non-covalently immobilized on a reversibly soluble-insoluble polymer, Eudragit L-100, and an immobilization efficiency of 0.93 was obtained. The optimum pH of the free and immobilized enzyme was 6.5 to 7.0 and 7.0, respectively, while there was no change in optimum temperature between the free and immobilized α-galactosidase. The imm...

متن کامل

Bioaffinity Based Immobilization of Almond (Amygdalus communis) b-galactosidase on Con A-layered Calcium Alginate-cellulose Beads: Its Application in Lactose Hydrolysis in Batch and Continuous Mode

In this study, immobilization of partially purified almond (Amygdalus communis) β-galactosidase on Con A layered calcium alginate-cellulose beads was investigated. Immobilized β-galactosidase retained 72% of theinitial activity after crosslinking by glutaraldehyde. Both soluble and immobilized enzyme exhibited the samepH and temperature optima at pH 5.5 and 50ºC, respectively. Howev...

متن کامل

Comment on ‘‘Elucidating the binding efficacy of β-galactosidase on graphene by docking approach and its potential application in galacto-oligosaccharide production”

In a recent study, Satar et al. (Bioprocess Biosyst Eng 39:807-814, 2016) have reported "the synthesis and characterization of graphene for the immobilization of β-galactosidase for improved galacto-oligosaccharide (GOS) production". There are several issues in this study that must be commented.

متن کامل

Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization.

A novel β-galactosidase from Lactobacillus plantarum (LPG) was over-expressed in E. coli and purified via a single chromatographic step by using lowly activated IMAC (immobilized metal for affinity chromatography) supports. The pure enzyme exhibited a high hydrolytic activity of 491 IU/mL towards o-nitrophenyl β-D-galactopyranoside. This value was conserved in the presence of different divalent...

متن کامل

Galacto-oligosaccharides synthesis from lactose and whey by β-galactosidase immobilized in PVA.

The synthesis of galacto-oligosaccharides (GOS) by β-galactosidase immobilized in both polyvinyl alcohol (PVA) lenses and sol-gel carriers was studied and compared with the performance of the free enzyme. PVA-immobilized β-galactosidase retained 95 % of the initial activity after seven repeated uses and retained 51 % of the initial activity after 3 months of storage, while sol-gel-immobilized β...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2017